The adoption of the One Health approach in biodiversity-related projects requires an open and participatory discussion among all actors and stakeholders involved and affected by the project itself. The discussion will build on the results of project analysis, collaboratively planning how (principles) and where (gate entries) the One Health approach can be applied and identifying what (measures) can be done to ensure integration is optimal and relevant. The review team will lead the preparation of an action plan to ensure that fundamental factors (enabling conditions) are met and guide the operationalisation of the OH component at the human-animal-environment interface.

Enabling conditions determine the success of the OH integration in the project. Their accomplishment is necessary to create a suitable environment for sustainable and optimal collaborations and activities. The enabling conditions identified in the framework include a conducive political environment that encourages government and non-state actors across all relevant organisational levels to willingly collaborate; infrastructure, tools and processes that ease the sharing of data and enable the co-design of multisectoral interventions at the human-animal-environment interface; a detailed stakeholder mapping that allows the identification of strengths and potentials across different actors and promotes the establishment of valuable collaboration; and a meaningful investment that sustains the application of the One Health approach in new or existing project.

Measures are interventions or activities that are already implemented in the project and can allow building a One Health component within its scope. They enable the operationalisation of the One Health integration in an optimal and relevant way. The framework identifies eight measures, including Education and Awareness, Policy Development, Capacity Development, Collaborative Platforms, Community Engagement, Information Sharing, Surveillance and Early Warning, and Research.

Gate entries are thematic areas in which the project conducts activities or actions that have the potential to link into a OH approach. They represent real opportunities to integrate and transform project goals and One Health goals into a common goal. At the biodiversity-health nexus, the framework identifies five main key gate entries: Emerging Infectious Diseases and Zoonoses, Agriculture Production and Food Safety, Climate Change and Risk Reduction, Wildlife Trade and Consumption, and Biodiversity Conservation (including Nature-Based Solutions, Protected Areas, and Wildlife Management).

The first step of the analysis is to assess if the project is applying one or more OH principles within its scope of work. Seven OH principles, adapted from the literature, are used in the framework (i.e., multisectoral, transdisciplinary, participation, prevention, decentralisation, evidence-based, multi-scalar). Not all principles in the framework have equal value, with the multisectoral principle considered an essential component in the proposed tool. The reason for this is that the basis of One Health is the collaboration among different sectors. The framework allows multisectoral collaborations at any level, for example where an MoU has been signed between ministries, or at community level through the joint effort of village health workers, animal health volunteers and rangers.

Once formed, a review team will lead the analysis of the project and the potential integration of the One Health approach. It is key to initiate a discussion across sectors, identify opportunities of collaboration among stakeholders, and enable the co-design of One Health components that align with and push the project goals towards a holistic approach to the biodiversity-health nexus. The review team is multidisciplinary and should be made up of all the project owners, including institutional and development partners.

Partnerships with the provincial Taskforce, which oversaw the project's activities in general, and the provincial Agriculture Extension Center and Farmers’ Union helped achieve the expected results despite travel restrictions owing to COVID-19.

Partnerships with these local partners also led to the later integration of the project’s interventions into provincial partner’s programmes.

Financing support for multi-sector surveillance of zoonotic diseases in wildlife and along wildlife trade chains is essential to improve understanding of pathogen diversity, disease dynamics and potential risks posed by wildlife trade, to support evidence-based decision making, monitor emerging pathogens and support targeted future surveillance and mitigation investments

Building long-term partnerships and capacity for multi-sector surveillance of zoonotic diseases along wildlife trade chains promotes and supports effective One Health collaboration from the spillover frontlines to national and international policy makers, and improved understanding of pathogen diversity, disease dynamics and potential risks posed by wildlife trade. Platforms for regular multi-sector discussion between animal health, environment, enforcement, and human health sectors, as well as with and amongst international and multi-lateral partners, is essential for open discourse and sharing of information on the risks, challenges and opportunities for pandemic prevention. This is critical to improve cross- sectoral trust, understanding, communication and coordination to increase opportunities for effective legislative change and cultural shifts.

The sites are heterogeneous and have different degrees of degradation, resulting in different intervention needs and opportunities. It is crucial to understand which species appear in nature as groups and to design the planting design accordingly. Mother trees, flowering periods and storability of seeds need to be known and the planting has to be adapted according to suitable planting periods. In the nursery, the focus on good root systems of the seedlings is key, requiring appropriate nursing equipment, substrate and trained staff.