Localized Reference Library Development

The creation of a comprehensive, open-access DNA reference library of native plant and animal species was foundational to our solution. Recognizing that global databases lacked coverage for many Eastern Mediterranean species, we built the first Lebanese library encompassing plants, mammals, and now expanding to insects, birds, and fungi. This reference database improves the accuracy of DNA sequence matching and enables precise identification of species present in environmental samples. It also fills a critical regional data gap and facilitates ecological studies, biodiversity monitoring, and conservation planning. By publishing the library openly, we promote transparency, collaboration, and the potential for adaptation in similar biodiversity hotspots.

Strong institutional support from Saint Joseph University, collaboration with local taxonomists, and access to specimens were vital. Funding from initial grants allowed sequencing efforts. Commitment to open data principles ensured broad accessibility. Support from iBOL facilitated integration into global databases, enhancing utility and visibility.

Building a reliable reference library requires significant coordination between molecular scientists and taxonomists. Accurate species identification depends heavily on quality-verified voucher specimens and metadata. The process is time-consuming but indispensable for meaningful metabarcoding results. Sharing the library openly generated interest and collaboration but also highlighted the need for continuous updates and expansion to cover more taxa. Engaging local experts fostered ownership and increased the scientific credibility of the data, ensuring the library’s sustainability as a national resource.

Advanced DNA Barcoding and Metabarcoding Techniques:

DNA barcoding and metabarcoding are cutting-edge molecular techniques that allow precise identification of species from small biological samples such as animal scats. Barcoding targets a single species by sequencing a standard gene region, while metabarcoding amplifies multiple DNA markers simultaneously, enabling comprehensive analysis of complex mixtures. These methods provide detailed insights into animal diets, predator-prey relationships, and seed dispersal patterns without invasive sampling. In our solution, these techniques were adapted to the Lebanese ecological context, enabling high-throughput biodiversity assessment and revealing key interactions between fauna and flora. This approach overcomes limitations of traditional ecological surveys and opens new possibilities for monitoring biodiversity changes, especially in regions with scarce baseline data.

Access to high-throughput sequencing technology, expertise in molecular biology, and the availability of regional reference libraries enabled successful implementation. The collaboration with international experts, such as the Smithsonian Institution, ensured methodological rigor. The development of protocols tailored to local conditions and sample types was crucial for reliable results. Funding from FERI and MEPI provided the necessary resources to establish and scale the molecular workflows.

We learned that customizing DNA metabarcoding protocols to local ecological conditions is essential to maximize data accuracy. Establishing comprehensive reference libraries beforehand is critical for correct species identification. Early engagement with molecular experts and international partners accelerated technology transfer and improved quality control. We also discovered that noninvasive sampling methods, such as scat collection, can yield rich data but require strict protocols to avoid contamination. Finally, integrating these molecular tools with traditional ecological knowledge strengthens interpretation and practical application for restoration.

Connecting with Komodo: Building Place Attachment for Conservation Leadership

This building block focuses on cultivating place attachment—an emotional, cultural, and cognitive bond between youth and Komodo National Park. Through storytelling, field immersion, and reflective learning, students begin to see the park not just as a tourist destination, but as a vital part of their identity and future. The program employs experiential tools such as ranger-led nature walks, local legends, visual storytelling, and ecological mapping to foster deeper connections with the landscape and its inhabitants. These experiences help reposition the park from a backdrop of economic activity into a living, shared heritage. As students develop a sense of belonging and pride, their motivation to protect and advocate for the environment increases. This shift is crucial in transforming passive knowledge into active stewardship, inspiring long-term behavioral change and conservation leadership. Building place attachment ensures that youth understand both the ecological and emotional value of the park, anchoring their sense of responsibility in a place they call home.

Key enablers include the presence of committed park rangers who serve as mentors, local legends and cultural narratives that resonate with students, and access to Komodo National Park as an outdoor classroom. Collaborative partnerships with schools ensure curricular alignment and logistical support. The trust built between students and facilitators, paired with immersive activities in nature, significantly enhances students’ emotional connection to the park and their willingness to become stewards.

One powerful lesson is that connection precedes conservation. Students are more likely to care for and protect a place they feel emotionally and culturally bonded to. We also learned that place attachment cannot be forced—it must be earned through authentic, meaningful experiences. Building trust between facilitators and students takes time but is essential for success. Another insight is the importance of cultural relevance: stories, language, and examples drawn from local contexts deepen resonance and memory. Finally, place attachment is not only about nostalgia or pride—it can be a powerful driver for transformation. When students feel ownership of Komodo National Park, they begin to see conservation not as someone else’s job, but as a personal responsibility. This shift is what transforms students from observers into advocates, and classrooms into launchpads for future conservation leaders.

Simplified conservation actions

A leading cause for people not adopting pro-environmental behaviours is low self-efficacy. That is, they do not believe they can employ the action themselves. Therefore, at the Station we simplified key conservation actions which UK and Jersey consumers can do to support wild Sumatran orangutans. One of these steps was to download the PalmOil Scan app, which enables consumers to identify companies actively sourcing sustainable palm oil. This then allows people to make informed decisions as to which products they buy, and as a result which companies they support. 

  • Breaking down conservation actions in to easy, achievable steps
  • Providing opportunities to see the conservation behaviour in action (e.g. provision of empty packaging with barcodes to scan using the app)
  • Provide an easy way for audiences to get involved (e.g. provide a QR code for downloading an app)
  • Staff/volunteers present allowed visitors to ask specific questions and solidify their understanding

Where we were hosting the Station there was limited access to Wi-Fi and mobile signal and so downloading the app, especially for overseas visitors, sometimes proved difficult. Setting up a portable network provider for visitors to connect to could avoid this issue. Alternatively, leaflets with information and links to the app for visitors to photograph or take away with them may be a more practical solution for visitors to take action at a more convenient time. However, this would make evaluating the number of visitors successfully downloading the app difficult. 

Collaborative planning

Throughout the development and delivery of the Station, mammal keepers at Jersey Zoo were consulted regularly to ensure the resources were both accurate and not disruptive to the animals (e.g. the sound button recordings). This collaboration also allowed the keepers, who were enthusiastic of the idea, to share details of their daily routines and anecdotes of the orangutans which could then be incorporated into the Station's storytelling element. The keepers also helped supply materials and biofacts to use at the Station, along with ideas on how to use them effectively. These elements, including some real orangutan teeth, significantly increased the attraction power and impact of the Station. Feedback from volunteers and those running the Station was also encouraged to ensure the resources met the needs of the educators, as well as the audience. 

  • Building strong relationships with the animal keepers, volunteers and other contributors for sharing ideas, information and feedback
  • Working closely with the appropriate keepers to ensure resources are factual and appropriate
  • Collecting anecdotes from keepers and volunteers to enhance storytelling and emotional connections between visitors and the orangutans at the zoo 
  • Encourage feedback from volunteers, with appropriate suggestions being actioned quickly

Before running the Station, each volunteer was invited to a training session and provided with a handbook with anecdotes and information on the history of orangutans at Jersey Zoo. This training worked well to ensure all educators had a baseline knowledge. However, having a shorter, easily accessible fact sheet with information such as orangutan's habitat, lifespan and key facts about the zoo's individuals proved to be a necessary addition to the Station, as suggested by the volunteers. 

Tailoring of resources

The resources at the Station are organised into five themes: 'Sounds & Smells', 'How you can help', 'Could you be an orangutan?', 'What does an orangutan feel like?' and 'Orangutan Observations'. However, the flexibility of the resources means they can easily be mix-and-matched and tailored to both the audience's curiosity and the staff/volunteer's expertise. That is, no resource is dependent on any other. Furthermore, resources from each of the themes can easily be connected together. 

For example, the armspan ropes, handprint and sound buttons could be combined to talk about orangutans' arboreal lifestyle and how they have adapted to their habitat. This can then lead to a conversation about the destruction of their habitat and the need for sourcing sustainable palm oil. Alternatively, the handprint and grip strength dynamometer can be used to create a sense of awe and subsequently a memorable impression on the visitor. 

  • Independent resources which can be connected via a meaningful, awe-inspiring story
  • Resources relatable to your audience (e.g. the orangutan's hand print can be compared with a visitors' own)
  • Staff / volunteers leading the Station can change the arrangement of resources depending on their strengths and audience
  • New staff / volunteers are trained on what resources are on offer and a variety of ways they can use them effectively

Clear organisation is needed when storing the resources to ensure each volunteer / staff member can set-up quickly and easily retrieve relevant resources when talking to a visitor. We also found that constant staffing of the Station can be costly, in terms of time, and so may not always be possible, especially if volunteers are in short supply. 

Interactive & sensory-based resources

The different sensory-based elements of the Investigation Station offer alternative pathways for visitors to access conservation messages, away from traditional signage. Interactive methods, such as this, allows information to be conveyed in a memorable and, therefore, more impactful way. It also meant visitors with a variety of Special Educational Needs and Disabilities (SEND) were still able to engage with the station and learn something new. 

The Station's resources covered most of the five human senses, with each resource conveying a different educational message. Additionally, the sensory items were all relatively easy to obtain or make, meaning production costs remained low. 

Touch

  • Worn gardening glove (to represent an orangutan’s skin texture)
  • Combed out rope (to mimic hair)

Smell

  • Smell cubes (e.g. durian fruit)

Hearing

  • Sound buttons with relevant recordings (e.g. orangutan long call)

Sight

  • Coloured photos (e.g. to show orangutan's habitat and the impact of palm oil plantations)

Despite the consideration for visitors with SEND, some children with autism found the sounds and smells to be overwhelming. In these cases, we removed these elements from the Station and used different resources to demonstrate our points. Having the staff member or volunteer present at the Station meant we were able to quickly adapt the Station to each visitor's learning needs. 

Dissemination - To promote the application of IoT technology on wetland management

To promote the application of IoT technology on wetland management, this research project and experience learnt has been shared throughout the project by:  

  • Organizing webinars for conservation managers and wetland stakeholders  
  • Installing outdoor interpretation panels in MPNR  
  • Promoting through social media platform and media engagement  
  • Producing promotional video  
  • Publishing technical report of the project  
  • Organizing public and school tours to introduce the application of IoT technology in wetland management at MPNR 

3 seminar/webinar of IoT application on wetland management were held. They were attended by over 120 participants including practitioners from over 30 managed sites across the globe, National Forestry and Grassland Administration from mainland China, local government departments, NGOs, public and private institutions.

Interpretation panels were installed inside MPNR telling the visitor the purpose of the 3 types of IoT sensors.

Several posts were published through social media, and media was engaged to let the public know the major components of the project and the importance of wetland and its proactive management.

1 promotional video was produced and published telling the public what we have done and achieved in this 2-years project

1 Technical Report on IoT Application for Wetland Conservation in Mai Po Nature Reserve was created and published providing recommendations for future IoT deployments in similar wetland environments, highlighting the scalability and replicability of this approach.   

Public and school tours to MPNR were held to introduce the application of IoT technology in wetland management at MPNR.

This dissemination phase is crucial for ensuring that the results and deliverables of this project are shared with stakeholders and the wider audience. Here are some lessons learned:

  • Identifying and targeting the right audience is essential. This includes internal stakeholders, other projects, and the broader community.
  • Various methods can be used for dissemination, such as reports, presentations, workshops, social media, and publications.
  • Dissemination should be planned throughout the project lifecycle, with specific activities scheduled at different phases to maximize impact.
  • Engaging stakeholders through dissemination really helps in gathering feedback, fostering collaboration, and ensuring the project's sustainability
  • The importance of thorough documentation and clear reporting. This ensures that lessons learned are accessible and can be referred to in future projects
  • The activities done in dissemination phase can be used as an opportunity to identify areas for improvement in project processes and methodologies

 

 

Foundation - Consultation with stakeholders, identify and prioritize suitable device, platform and network to be applied at MPNR for efficient wetland monitoring

Goal: To lay the foundation for integrating I&T solutions into traditional wetland management

Objectives: 

 - To introduce three proposed IoT applications to wetland stakeholders and I&T sectors 

 - To consult wetland stakeholders and I&T sectors for their views and comments 

 - To review potential IoT applications beneficial to the management of MPNR 

 - To identify and integrating relevant IoT solutions for gei wai operation and wetland research into MPNR  

 

 

 

 

Studies were conducted to identify and prioritize suitable device, platform and network to be applied at MPNR for efficient wetland monitoring by:  

Consultation with wetland stakeholders:

  • Consultation with Mai Po Management Committee (MPMC), which comprises of representatives from Agriculture, Fisheries and Conservation Department, Hong Kong Bird Watching Society, academics, independent wetlands experts and ornithologists.  Obtained advice on the proposed IoT application. 
  • Consultation with local village communities. Their input helped align the project with community values and needs.
  • Consultation with innovation and technology sectors. Technical advice obtained on the selection of proposed IoT applications, transmission network system and deployment requirements from Electrical and Mechanical Services Department, Innovation and Technology Commission, member company of Incu-Tech Programme, Hong Kong Science & Technology Park.  

Review of potential IoT applications in wetland conservation:

  • A Technical Review Summary of Potential IoT Application was created (as attached)

Views and suggestions from MPMC are summarized as follows: 

  • Given that I&T solutions could be expensive, compromise between data sampling frequency and cost has to be made
  • Selection of locations for sensors deployment provides detailed information for habitat management
  • Quality control of data is crucial 
  • The real-time, automated IoT monitoring system enhances efficiency of data collection. The data can correlate with other scientific researches
  • Deployment of sensors might visually affect the natural landscape of wetlands

Views and suggestions from I&T sector are summarized as follows:

  • The proposed three applications are feasible 
  • Scope of service has to be clearly defined. This might include a execution plan, application implementation service, technology consultation service, vendor management service, on-site installation, platform establishment, data analytic service, maintenance service 
  • The platform should be able to capture data, establish data pipeline and integration and perform data analysis 
  • Functional requirement for the platform such as user access, building information model (BIM), alert, data streaming and dashboard analytics should be clearly stated
  • Technical requirements for the platform such as computer specification for operating the platform, BIM format and capacity, data security should be clearly stated 

 

Key Components (Building Blocks)

1. Integrated Technical and Traditional Knowledge

Mussel production combines modern aquaculture techniques with the traditional knowledge of local fishermen. This integration facilitates the acceptance of the practice and its alignment with community routines. 

Enabling factors: proximity to the sea and familiarity with tidal cycles. Lessons learned: valuing local knowledge increases engagement and the effectiveness of adopted practices.

2. Cultivation System with Longlines

Mussel farming in Machangulu involves several meticulous steps that require specific materials and aquaculture techniques. Long ropes, 50 meters long on which sachets with mussel seeds are hung, are prepared and are held fixed by a concrete weight to serve as an anchor on the seabed, a procedure that keeps the longlines stretched and where the signaling buoys are placed. This system keeps the mollusks submerged even during low tides and requires ongoing maintenance.

The main materials include nets, ropes better known as longlines, buoys, mussel seeds, hand tools and sea transport. The longlines, cotton nets are sewn in the form of sachets, where the mussel seeds are placed, with initial dimensions between 1 and 1.5 inches (small mussels). This system ensures that the mussels remain submerged in water, even during the lowest tides, during cycle lasts eight months.

Availability of basic materials and technical support. 

 

 Continuous technical training is essential to ensure good management practices and guarantee the quality of the final product;

The valorization of local knowledge contributes to greater community ownership of technology;

The absence of clear strategies for access to foreign markets still limits the growth and profitability of production.