Advanced DNA Barcoding and Metabarcoding Techniques:
DNA barcoding and metabarcoding are cutting-edge molecular techniques that allow precise identification of species from small biological samples such as animal scats. Barcoding targets a single species by sequencing a standard gene region, while metabarcoding amplifies multiple DNA markers simultaneously, enabling comprehensive analysis of complex mixtures. These methods provide detailed insights into animal diets, predator-prey relationships, and seed dispersal patterns without invasive sampling. In our solution, these techniques were adapted to the Lebanese ecological context, enabling high-throughput biodiversity assessment and revealing key interactions between fauna and flora. This approach overcomes limitations of traditional ecological surveys and opens new possibilities for monitoring biodiversity changes, especially in regions with scarce baseline data.
Access to high-throughput sequencing technology, expertise in molecular biology, and the availability of regional reference libraries enabled successful implementation. The collaboration with international experts, such as the Smithsonian Institution, ensured methodological rigor. The development of protocols tailored to local conditions and sample types was crucial for reliable results. Funding from FERI and MEPI provided the necessary resources to establish and scale the molecular workflows.
We learned that customizing DNA metabarcoding protocols to local ecological conditions is essential to maximize data accuracy. Establishing comprehensive reference libraries beforehand is critical for correct species identification. Early engagement with molecular experts and international partners accelerated technology transfer and improved quality control. We also discovered that noninvasive sampling methods, such as scat collection, can yield rich data but require strict protocols to avoid contamination. Finally, integrating these molecular tools with traditional ecological knowledge strengthens interpretation and practical application for restoration.