Es importante contar con una metodología que responda a las características y comportamiento del cultivo. Que permita establecer lotes, transeptos, árboles -ver BB 3- y tratamientos para la evaluación del efecto del servicio de polinización de manera objetiva. En el caso del marañón, se establecieron dos montajes por árbol: 1) Control absoluto: exclusión total de polinizadores con mallas que encierran las ramas seleccionadas, 2) Polinización: ramas marcadas y totalmente descubiertas. Al finalizar el periodo de floración y posterior fructificación, se comparan las diferencias entre los dos montajes para a) porcentaje (%) de cuajado de frutos, b) tamaño y peso de la manzana, c) tamaño y peso de la nuez, d) tamaño y peso de la almendra.

Al momento de realizar ensayos en campo, se busca que los cultivos a evaluar tengan la mayor similitud para que los resultados sean más precisos. Por eso la distancia a centro urbano, la distancia a bosque nativo, la cercanía a fuentes hídricas, la variedad clonal y la edad de los árboles son variables que se deben considerar al momento de seleccionar las fincas. Así como la selección de colmenas con reina de buena postura y con alto interés de pecoreo (conducta de abejas obreras para salir a recolectar polen y néctar de las flores). 

Es importante que las personas involucradas entiendan los principios de la polinización; ¿qué es la polinización gestionada?, ¿qué implica?, ¿cuáles son los posibles riesgos y los resultados esperados? Para esto se realizaron una serie de capacitaciones.

El nivel de apropiación e interés en el proyecto es un factor determinante para su desarrollo y continuidad. Toda la información es valiosa y las partes involucradas deberían tener claridad desde lo que es un calendario floral y las características de floración del cultivo, hasta la estructura y funcionalidad de la abeja. Asumir que las personas ya cuentan con cierto conocimiento puede generar accidentes o resultados poco satisfactorios en la polinización gestionada.

The ABS project has been widely promoted throughout Guangxi, with about 50 promotional activities, 20 training sessions and related meetings, and more than 10,000 participants online and offline, to help with Guangxi ABS legislation and benefit-sharing.

Guangxi negotiated and implemented agreements on access to genetic resources and related traditional knowledge and benefit-sharing of Siraitia grosvenorii and Camellia nitidissima in Guilin and Fangchenggang, promoting the signing of more than 10 benefit-sharing agreements between enterprises and farmers in demonstration areas through measures such as value-insured recovery, benefit sharing, technology training, and enhanced resource protection. From 2019 to 2021, the campaign benefited more than 5,000 farmers in the major producing areas of Siraitia grosvenorii and Camellia nitidissima, helping nearly 300 poor households escape poverty.

The protection for wild genetic resources has been formalized, and 20 protection sites for chrysantha have been constructed; guidance is provided for demonstration enterprises to expand the nursery of Siraitia grosvenorii and Camellia nitidissima, which cover an area of 2,000 square metres and 500 square metres respectively.

The project team investigated, collected, and summarized the protection, development, utilization, and benefit-sharing of 30 exemplar genetic resources in Guangxi, carried out case investigations and research on the development and utilization of traditional knowledge related to genetic resources of five ethnic minorities in Guangxi Zhuang, including Zhuang, Yao, Maonan, Mulao and Jing ethnic groups.

Guangxi establishes a cross-sectoral coordination and cooperation mechanism and a stakeholder coordination mechanism.  A management mechanism for access and benefit sharing of biogenetic resources is established under the comprehensive governance of the department in charge of ecology and environment and the supervision of the departments related to development and reform, agriculture and rural areas, forestry, etc. The project management is carried out at the provincial, municipal, and county levels so joint efforts can be made in ensuring the access and benefit-sharing of biological heritage resources.

Since no population increase has been observed two years after the threat mitigation actions, in 2014 we launched a four-year restocking project aimed at increasing the population size.  
The project involves the collection in the wild of a fraction (30-40%) of Bombina pachypus population at the egg stage, their development in captivity and the subsequent release of metamorphs in the same site of collection.

All the ex-situ management of toads was implemented at Fondazione Bioparco facilities where individuals were kept until the ventral coloration pattern is fully defined so that they can be photographically marked for future identification and demographic studies in the wild.

Overall, 67 young individuals were released (20 in 2014, 19 in 2015, 16 in 2016 and 12 in 2017).

Post-release monitoring revealed that the toad recapture rate was highly variable over the years. In 2018, we re-captured a total of 21 individuals: 10 released in 2014, two in 2015, four in 2016 and five in 2017. The pristine population remained stable (13 individuals) with few new animals recruited and some losses. At the end of 2018, a net increase of 21 individuals released plus some from natural recruitment allowed the original population size to double.

Before any concrete conservation action that involve traslocation of individuals (with or without ex-situ reproduction) an assessment of the occurring threats and their mitigation/eradication is mandatory for the success of the actions.

Since the main threats observed for the species at the study site were the high risk of pond desiccation in the early stage of the breeding season (i.e., June) and alteration of the ponds by wild boars, two main conservation actions were carried out in 2012 to mitigate them: 
- four additional pools at each site fed by perennial springs were constructed to extend the hydro period from March to October;
- each puddle was fenced to prevent wild boars from using them for drinking and bathing.