Capacity Building and Regional Training Programs

Building local and regional expertise in DNA barcoding and metabarcoding is vital for sustainable biodiversity conservation. Supported by BBI-CBD funding, our training programs target conservation practitioners from Lebanon, Tunisia, Côte d'Ivoire, and Jordan, including those without prior molecular biology experience. These hands-on workshops cover sample collection, laboratory techniques, data analysis, and interpretation, empowering participants to independently apply molecular tools in their contexts. Capacity building democratizes access to innovative technologies, fosters regional collaboration, and ensures continuity beyond the project lifecycle.

Funding from BBI-CBD and institutional backing by Saint Joseph University enabled program development. Experienced trainers and tailored curricula accommodate diverse backgrounds. Regional participant selection promotes cross-country knowledge exchange. Ongoing support and follow-up strengthen learning outcomes.

We learned that successful capacity building requires flexible training models that accommodate participants’ varied expertise. Hands-on practice combined with theoretical knowledge improves retention. Establishing a regional network fosters peer learning and collaboration. Follow-up support and refresher sessions are important for sustained impact. Training must be paired with accessible resources and tools to enable real-world application. Engaging trainees as future trainers multiplies benefits and contributes to national and regional self-sufficiency in biodiversity monitoring.

Stakeholder Engagement and Knowledge Mobilization

Meaningful engagement with Lebanese Ministries of Environment and Agriculture, local NGOs, practitioners, and communities ensured that scientific insights informed policy and restoration practices. By communicating findings clearly and collaboratively, we helped integrate molecular data into the National Biodiversity Action Plan. Awareness campaigns targeted schools, universities, farmers, and land managers, raising understanding of the ecological roles animals play in forest regeneration. This knowledge mobilization builds local ownership, promotes evidence-based decision-making, and bridges science with societal needs for long-term ecosystem resilience.

Strong relationships with government agencies and NGOs fostered trust. Clear, accessible communication materials and workshops facilitated understanding. Involvement of local communities ensured relevance. Institutional support allowed integration into national plans. Funding enabled outreach and awareness activities.

Effective stakeholder engagement requires ongoing dialogue and tailored communication strategies to diverse audiences. We found that combining scientific rigor with accessible language bridges the science-policy-practice gap. Early inclusion of ministries and NGOs increases uptake of results. Awareness campaigns are essential to foster behavioral change and highlight the often-overlooked role of animals in ecosystem restoration. Sustained collaboration ensures findings influence policy and land management decisions. We also learned that participatory approaches empower communities, ensuring solutions are socially accepted and sustainable

Strategic International and Academic Partnerships

Partnerships with the Smithsonian Institution, iBOL, and Saint Joseph University have been central to our project’s success. The Smithsonian provided advanced expertise in metabarcoding methodology and quality assurance, enabling rigorous application of DNA analysis. iBOL supports the expansion of barcoding efforts, particularly for insects, linking our regional data to global biodiversity initiatives. Saint Joseph University leads research implementation and capacity building, ensuring regional ownership and continuity. These collaborations combine global knowledge with local ecological and institutional context, enabling innovation and scalability.

Long-term collaborative relationships, shared scientific goals, and mutual trust were key. International funding and technical assistance fostered knowledge exchange. The presence of a dedicated local research team facilitated communication and implementation. Shared commitment to open data and capacity building strengthened partnerships.

Strong partnerships require continuous communication, respect for local contexts, and clear roles. International collaboration accelerates technology transfer but must be coupled with local capacity building to ensure sustainability. We learned the importance of balancing global scientific standards with regional ecological realities. Formal agreements and joint planning helped align expectations. Integrating diverse expertise—from molecular biology to ecology and policy—enhanced project impact. Finally, these partnerships opened avenues for future research and expanded conservation networks.

Localized Reference Library Development

The creation of a comprehensive, open-access DNA reference library of native plant and animal species was foundational to our solution. Recognizing that global databases lacked coverage for many Eastern Mediterranean species, we built the first Lebanese library encompassing plants, mammals, and now expanding to insects, birds, and fungi. This reference database improves the accuracy of DNA sequence matching and enables precise identification of species present in environmental samples. It also fills a critical regional data gap and facilitates ecological studies, biodiversity monitoring, and conservation planning. By publishing the library openly, we promote transparency, collaboration, and the potential for adaptation in similar biodiversity hotspots.

Strong institutional support from Saint Joseph University, collaboration with local taxonomists, and access to specimens were vital. Funding from initial grants allowed sequencing efforts. Commitment to open data principles ensured broad accessibility. Support from iBOL facilitated integration into global databases, enhancing utility and visibility.

Building a reliable reference library requires significant coordination between molecular scientists and taxonomists. Accurate species identification depends heavily on quality-verified voucher specimens and metadata. The process is time-consuming but indispensable for meaningful metabarcoding results. Sharing the library openly generated interest and collaboration but also highlighted the need for continuous updates and expansion to cover more taxa. Engaging local experts fostered ownership and increased the scientific credibility of the data, ensuring the library’s sustainability as a national resource.

Advanced DNA Barcoding and Metabarcoding Techniques:

DNA barcoding and metabarcoding are cutting-edge molecular techniques that allow precise identification of species from small biological samples such as animal scats. Barcoding targets a single species by sequencing a standard gene region, while metabarcoding amplifies multiple DNA markers simultaneously, enabling comprehensive analysis of complex mixtures. These methods provide detailed insights into animal diets, predator-prey relationships, and seed dispersal patterns without invasive sampling. In our solution, these techniques were adapted to the Lebanese ecological context, enabling high-throughput biodiversity assessment and revealing key interactions between fauna and flora. This approach overcomes limitations of traditional ecological surveys and opens new possibilities for monitoring biodiversity changes, especially in regions with scarce baseline data.

Access to high-throughput sequencing technology, expertise in molecular biology, and the availability of regional reference libraries enabled successful implementation. The collaboration with international experts, such as the Smithsonian Institution, ensured methodological rigor. The development of protocols tailored to local conditions and sample types was crucial for reliable results. Funding from FERI and MEPI provided the necessary resources to establish and scale the molecular workflows.

We learned that customizing DNA metabarcoding protocols to local ecological conditions is essential to maximize data accuracy. Establishing comprehensive reference libraries beforehand is critical for correct species identification. Early engagement with molecular experts and international partners accelerated technology transfer and improved quality control. We also discovered that noninvasive sampling methods, such as scat collection, can yield rich data but require strict protocols to avoid contamination. Finally, integrating these molecular tools with traditional ecological knowledge strengthens interpretation and practical application for restoration.

Foundation - Consultation with stakeholders, identify and prioritize suitable device, platform and network to be applied at MPNR for efficient wetland monitoring

Goal: To lay the foundation for integrating I&T solutions into traditional wetland management

Objectives: 

 - To introduce three proposed IoT applications to wetland stakeholders and I&T sectors 

 - To consult wetland stakeholders and I&T sectors for their views and comments 

 - To review potential IoT applications beneficial to the management of MPNR 

 - To identify and integrating relevant IoT solutions for gei wai operation and wetland research into MPNR  

 

 

 

 

Studies were conducted to identify and prioritize suitable device, platform and network to be applied at MPNR for efficient wetland monitoring by:  

Consultation with wetland stakeholders:

  • Consultation with Mai Po Management Committee (MPMC), which comprises of representatives from Agriculture, Fisheries and Conservation Department, Hong Kong Bird Watching Society, academics, independent wetlands experts and ornithologists.  Obtained advice on the proposed IoT application. 
  • Consultation with local village communities. Their input helped align the project with community values and needs.
  • Consultation with innovation and technology sectors. Technical advice obtained on the selection of proposed IoT applications, transmission network system and deployment requirements from Electrical and Mechanical Services Department, Innovation and Technology Commission, member company of Incu-Tech Programme, Hong Kong Science & Technology Park.  

Review of potential IoT applications in wetland conservation:

  • A Technical Review Summary of Potential IoT Application was created (as attached)

Views and suggestions from MPMC are summarized as follows: 

  • Given that I&T solutions could be expensive, compromise between data sampling frequency and cost has to be made
  • Selection of locations for sensors deployment provides detailed information for habitat management
  • Quality control of data is crucial 
  • The real-time, automated IoT monitoring system enhances efficiency of data collection. The data can correlate with other scientific researches
  • Deployment of sensors might visually affect the natural landscape of wetlands

Views and suggestions from I&T sector are summarized as follows:

  • The proposed three applications are feasible 
  • Scope of service has to be clearly defined. This might include a execution plan, application implementation service, technology consultation service, vendor management service, on-site installation, platform establishment, data analytic service, maintenance service 
  • The platform should be able to capture data, establish data pipeline and integration and perform data analysis 
  • Functional requirement for the platform such as user access, building information model (BIM), alert, data streaming and dashboard analytics should be clearly stated
  • Technical requirements for the platform such as computer specification for operating the platform, BIM format and capacity, data security should be clearly stated 

 

Deployment - Partnership with local Government Department - Electrical and Mechanical Services Department (EMSD) for the utilization of Government-Wide IoT Network (GWIN)

Partnership with EMSD allows us to utilize GWIN network as a transmission layer for all sensors installed for this project at Mai Po Nature Reserve (MPNR) with no network recurring cost required.

The technology offers several benefits that make it well-suited for wireless data transmission in IoT applications. Its long-range capabilities allow data to be transmitted over a distance, making it ideal for vast rural areas like MPNR. Furthermore, LoRa’s efficient power consumption extends the battery life of battery-powered devices. LoRa data transmission also ensures the confidentiality and integrity of the data through secure protocols.

In this project, four GWIN LoRa gateways were installed by EMSD within Mai Po Nature Reserve (MPNR). The sensors installed at MPNR are connected to gateways via the low-power and private LoRa network and eventually connected back to the GWIN backend via the 4G network. Besides these four gateways specially installed for this project, other GWIN gateways near MPNR can further secure data transmission reliability. 

In return, these four gateways can also help receiving signal from the sensors installed by various government departments in the surrounding area. 

Traditional sensors would each require a 4G connection to connect to central servers. In the GWIN network, sensors are connected to gateways via the low power and private LoRa (Long Range) network. The network with low power consumption reduces the cost and complexity of installing the sensors and improves the security of the system and data without the need of using a third-party network.

 

 

Pursuing strong partnership with EMSD allows us to more efficiently tackle challenges we are facing at MPNR (limited access to internet and city power) through their well-established network and support. 

Building Block 1 Field Research & User Insights: Understanding Menstrual Product Access and Preferences in Nepal

This building block outlines the findings and methodology of a nationwide field study conducted in 2022, which informed the Sparśa Pad Project. The research examined menstrual product usage, access, stigma, and user preferences among 820 Nepali women and adolescent girls in 14 districts across all seven provinces.

Using a structured face-to-face interview approach, the team employed ethically approved questionnaires administered by culturally rooted female research assistants. This method ensured trust, context sensitivity, and accurate data collection across diverse communities. The interviewers were trained in ethical protocols and worked in their own or nearby communities, thereby strengthening rapport and enhancing their understanding of local norms, power relations, and languages.

Key findings revealed a high reliance on disposable pads (75.7%) and ongoing use of cloth (44.4%), with product preferences strongly shaped by income, education, and geography. Respondents prioritized absorbency, softness, and size in menstrual products. While 59% were unfamiliar with the term “biodegradable,” those who understood it expressed a strong preference for compostable options, over 90%. Importantly, 73% of participants followed at least one menstrual restriction, yet 57% expressed positive feelings about them, seeing them as tradition rather than purely discriminatory.

These findings directly shaped the design of Sparśa’s compostable pads, informed the user testing protocols, and guided the development of targeted awareness campaigns. The accompanying link and PDFs include a peer-reviewed research article co-authored by the team and supervised by Universidade Fernando Pessoa (Porto, Portugal), as well as informed consent forms, a statement of confidentiality, and a research questionnaire. These documents are provided for practitioners' reference or replication purposes.

Why this is useful for others:

For Nepali organizations and local governments:

  • The study provides representative national data to inform product design, pricing strategies, and outreach campaigns.
  • It reveals regional, ethnic, and generational differences in attitudes that are essential for localized intervention planning.
  • The questionnaire is available in Nepali and can be adapted for school surveys, municipal assessments, or NGO projects.

For international actors:

  • The research demonstrates a replicable, ethical field methodology that balances qualitative insight with statistically relevant sampling.
  • It offers a template for conducting culturally sensitive research in diverse, low-income settings.
  • Key insights can guide similar product development, health education, and behavior change interventions globally.

Instructions for practitioners:

  • Use the attached PDFs as templates for conducting your own baseline studies.
  • Adapt the questions to reflect your region’s cultural and product context.
  • Leverage the findings to avoid common pitfalls, such as overestimating awareness of biodegradable products or underestimating positive views on restrictions.
  • Use the structure to co-design products and testing tools that truly reflect end-user needs.

 



 

  • Long-term engagement of NIDISI, a NGO with operational presence in Nepal, enabled trust-based access to diverse communities across the country.
  • Partnerships with local NGOs in regions where NIDISI does not operate directly were essential to extend geographic reach. In Humla, one of Nepal’s most remote districts, the entire research process was carried out by a trusted partner organization.
  • Pre-research networking and stakeholder consultations helped NIDISI refine research tools, adapt to local realities, and align with the expectations of communities and local actors.
  • Research assistants were female community members selected through NIDISI’s existing grassroots networks and recommendations from NGO partners, ensuring cultural sensitivity, linguistic fluency, and local acceptance.
  • Field research relied on ethically approved, pre-tested questionnaires, with interviews conducted in multiple local languages to ensure inclusivity and clarity.
  • Interviews were conducted face-to-face and door-to-door, prioritizing trust and participant comfort in culturally appropriate ways.
  • The study included a demographically diverse sample, representing various ethnic, educational, religious, and economic groups, strengthening the representativeness and replicability of the findings.
  • Academic collaboration with Universidade Fernando Pessoa (Portugal), where the research formed part of a Master's thesis by a NIDISI team member, ensuring methodological rigor and peer-reviewed oversight.
  • Language and cultural barriers can compromise data accuracy; working with local female facilitators from the same communities was essential to ensure comprehension, trust, and openness.
  • Social desirability bias limited the honesty of some responses around menstrual stigma. Conducting interviews privately and individually helped mitigate this, especially when discussing taboos or product usage.
  • The combination of quantitative surveys with qualitative methods (open-ended questions, observations, respondent quotes) enriched the dataset and provided both measurable and narrative insights.
  • Flexibility in logistics was crucial. Travel difficulties, seasonal factors, and participant availability—especially in rural and remote areas—required adaptable timelines and contingency planning.
  • Respecting local customs and religious norms throughout the research process was vital for ethical engagement and long-term acceptance of the project.
  • Training research assistants thoroughly not only on tools, but also on the ethical handling of sensitive topics, significantly improved the reliability and consistency of data collected.
  • Some communities initially associated the topic of menstruation with shame or discomfort, and pre-engagement through trusted local NGOs helped build the trust necessary for participation.
  • Pilot-testing the questionnaire revealed linguistic ambiguities and culturally inappropriate phrasing, which were corrected before full deployment—this step proved indispensable.
  • Remote district such as Humla required alternative model: relying fully on local NGO partners for data collection proved both effective and necessary for reaching hard-to-access populations without extensive budget burden.
  • Participant fatigue occasionally affected the quality of responses in longer interviews; reducing the number of questions and improving flow would significantly improve participant engagement.
  • Engaging with younger respondents, especially adolescents, required different communication strategies and levels of explanation than with older adults. Age-sensitive adaptation improved both participation and data depth.
  • Documentation and data organization during fieldwork (e.g. daily debriefs, note-taking, photo documentation, secure backups) was essential for maintaining data quality and enabling follow-up analysis.
Agreement on Designating and Demarcating Restoration Sites

To ensure effective restoration, project leaders worked with the community to designate and physically demarcate specific areas for intervention. This included mapping the landscape to identify priority areas for rangeland restoration, addressing concerns over land ownership, and ensuring community consensus on where boundaries would be drawn. The collaborative designation process allowed for clear agreements on how the land would be used, while physical markers helped prevent future conflicts over land use and maintain restoration areas.

  • Community-Driven site identification      and Planning: Engaging the community in mapping exercises fostered a shared understanding of the landscape and prioritized areas needing urgent intervention.
  • Clear Boundary Markings: Physically marking boundaries (e.g., with fencing or natural markers) and border patrols by community scouts provided clear, visible indicators of designated restoration areas, reducing misunderstandings over land use.
  • Consensus-Building Processes: Reaching community-wide agreements on site designation      increased local commitment to respecting and protecting these areas.
  • Clear Boundaries Prevent Conflict: Physically marked boundaries reduce potential disputes over land use, ensuring that restoration areas remain protected.
  • Local Input Enhances Relevance and Suitability: Community involvement in site selection increases relevance, as local knowledge helps identify areas that are both ecologically valuable and socially acceptable for restoration.
  • Ownership Through Shared Decision-Making: Engaging the community in boundary designation fosters a sense of ownership, increasing commitment to maintaining and protecting these restoration sites.
Organization of Community Meetings with Landowners (Local Barazas)

Community meetings, or barazas, served as the primary platform for engagement with landowners to discuss and align on proposed restoration interventions across 150 hectares of rangeland. These meetings were designed as participatory sessions where local Maasai landowners could voice their concerns, ask questions, and gain clarity on how the project would impact their land use. The barazas were instrumental in fostering transparent communication between project implementers and local stakeholders, allowing for an inclusive decision-making process that respected Maasai traditions and community dynamics.

  • Culturally Respectful Engagement Methods: Conducting barazas, a traditional community meeting format, allowed project leaders to approach discussions in a culturally appropriate manner.
  • Representation and Inclusion: Including landowners and community representatives ensured that diverse perspectives were heard and that decisions reflected the community's priorities.
  • Consistency and Follow-Up: Regular meetings provided a forum for ongoing dialogue and feedback, strengthening trust and adaptability to address any new concerns as the project progressed.
  • Transparency Builds Trust: Open and transparent discussions in barazas helped demystify the project’s goals, building trust and minimizing resistance among community members.
  • Community Ownership Leads to Better Alignment: When landowners are actively involved in the decision-making process, they are more likely to support and participate in the project, ensuring that interventions align with their land use priorities.
  • Iterative Feedback is Key: The regular nature of barazas provided continuous feedback, allowing the project to be responsive and adaptive to local needs and evolving challenges.